Bio 1406 Enzyme Report

1) ABSTRACT The purpose of this sample was to carry forth the bugger off of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the examine was also to understand the relationship between the change in absorbance with time and the enzyme answer direct. To perform the experiment, the class was divided into groups. apiece group took on one of the factors affecting the enzyme. Before ascendent the actual experiment, we apply the mixing tables that were given to us to peg down up the test tubes with the appropriate substances. We followed the same procedure for sepa strayly experiment variable (1-5). We then rid the cell of toxic hydrogen peroxide (H2O2) so the cell would not book damaged, as stated in our lab manual. For every experimental variable, test tube 1 was always the control. Once the solutions were bear down we mixed them and poured them into the cuvette and apply the spectrophotometer m achine to run the enzyme and determine its rate and its absorbance. When the actual experiment was done we all shared the results and graphed them. 2) intromission Before beginning the actual experiment we had to become familiar with a spectrometer machine and its functions.

It is basically an instrument used to measure the amount of light absorbed by a solution (Frankhauser, David B). As stated in our lab manual, the darker the solution, the great its absorbance. The reason why we used this machine was to figure break the relationship between the change in absorbance with time and the enzyme reaction rate. The enzyme reaction rate was determine! d when plots of absorbance versus time were constructed. The slope of all(prenominal) plot was related to the reaction rate. After running a pocket-size experiment to learn how to use the spectrophotometer, we learned or so the background information of enzymes. Enzymes are proteins that serve as atom smasher (Ophardt, Charles E.). By definition, a catalyst is a substance that initiates or accelerates...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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